BLUGEN
Polyplus
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상품 정보
상품 기본설명
Protein, antibody and peptide delivery reagent
상품 상세설명
| Reagent | PULSin® |
|---|---|
| Molecule delivered | Protein; Antibody; Peptide |
| Cell types | Adherent & suspension cells grown in presence of serum |
| Number of transfection | 0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates |
| Storage | Store PULSin® at 5 °C ± 3°C. Do not freeze. |
| Provided with | Hepes Buffer (20mM) for protein dilution; R-Phycoerythrin used as a positive control |
Summary
The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. For example, PULSin® enables you to study lethal proteins by controlling the level and time course of protein delivery into the cells. Similarly, delivery of blocking antibodies may provide additional information to traditional RNA interference experiments*. With PULSin® you can target intracellular proteins with antibodies in living cells without fixation step.
* G. Cassinelli et al. (2006). Inhibition of c-Met and prevention of spontaneous metastatic spreading by the 2-indolinone RPI-1, Mol Cancer Ther 5:9, 2388-2397.
Ordering information
| Reference number | Amount of reagent | Positive control (R-phycoerythrin) | Hepes dilution buffer |
|---|---|---|---|
| 101000010 | PULSin® 0.4 mL | 20 µg | 20 ml |
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